B Vitamins in Starchy and Sugary Maize Endosperms.

Lysine-Ketoglutarate Reductase in Normal and Opaque-2 Maize Endosperms'

Lysine-ketoglutarate reductase catalyzes the first step of lysine catabolism in maize (Zea mays L.) endosperm. The enzyme condenses L-lysine and a-ketoglutarate into saccharopine using NADPH as cofactor. It is endosperm-specific and has a temporal pattern of activity, increasing with the onset of kernel development, reaching a peak 20 to 25 days after pollination, and thereafter decreasing as t...

Linkage between Sweet-Defective and Sugary Endosperm in Maize.

PAGE INTRODUCTION.. ................................................................ 395 Additional data on the inheritance of sweet-defective. ............................... 395 Linkage between defective endosperm and sugary endosperm. ........................ 397 SUMMARY ....................................................................... 401 LITERATURE CITED.. . . . . . . . . . . . . . ....

Partial purification and characterization of lysine-ketoglutarate reductase in normal and opaque-2 maize endosperms.

Lysine-ketoglutarate reductase catalyzes the first step of lysine catabolism in maize (Zea mays L.) endosperm. The enzyme condenses l-lysine and alpha-ketoglutarate into saccharopine using NADPH as cofactor. It is endosperm-specific and has a temporal pattern of activity, increasing with the onset of kernel development, reaching a peak 20 to 25 days after pollination, and there-after decreasing...

The Segregation of Carbohydrates in Crosses between Waxy and Starchy Types of Maize.

Agrobacterium tumefaciens-mediated transformation of maize endosperm as a tool to study endosperm cell biology.

Developing maize (Zea mays) endosperms can be excised from the maternal tissues and undergo tissue/cell-type differentiation under in vitro conditions. We have developed a method to transform in vitro-grown endosperms using Agrobacterium tumefaciens and standard binary vectors. We show that both aleurone and starchy endosperm cells can be successfully transformed using a short cocultivation wit...